Products for Research Use Only
RegisterLogin

Human Programmed cell death 4 (PDCD4) ELISA Kit

CAT: 0749-KTE61278-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0749-KTE61278-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
PDCD4 encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.The deduced 458-amino acid protein has a calculated molecular mass of 50.6 kD. PDCD4 is an acidic protein with characteristics of a nuclear nonhistone protein. It contains 2 overlapping GxGxxG nucleotide-binding motifs, which are characteristic of protein kinases, but it does not have a kinase catalytic domain. PDCD4 also has several putative serine and threonine phosphorylation sites. Northern blot analysis detected transcripts of about 2.7 and 3.8 kb in HeLa cell total RNA.
Description
This Human Programmed cell death 4 (PDCD4) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD4.
CAS Number
7732-18-5
Product Name Alternative
PDCD4; RP11-348N5.4; H731; MGC33046; MGC33047; nuclear antigen H731; programmed cell death 4
Gene ID
27250
Accession Number
Q53EL6
Reactivity
Human
Tag
PDCD4
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Programmed cell death 4 (PDCD4) ELISA Kit has high sensitivity and excellent specificity for detection of Human PDCD4. No significant cross-reactivity or interference between Human PDCD4 and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Human Programmed cell death 4 (PDCD4) ELISA Kit employs a two-site sandwich ELISA to quantitate PDCD4 in samples. An antibody specific for PDCD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDCD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDCD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDCD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
PDCD4; RP11-348N5.4; H731; MGC33046; MGC33047; nuclear antigen H731; programmed cell death 4
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/Q53EL6
Calibration Range
Please inquire

Related Products

CatalogName
KTE61276-01Human Programmed cell death protein 6 (PDCD6) ELISA Kit
KTE61277-01Human Programmed cell death protein 5 (PDCD5) ELISA Kit
KTE62942-01ELISA kit for Human Programmed cell death protein 2 (PDCD2)
KTE61275-01Human Programmed cell death 6-interacting protein (PDCD6IP) ELISA Kit
KTE61279-01Human Programmed cell death 1 ligand 2 (PDCD1LG2) ELISA Kit
KTE61280-01Human Programmed Death 1 (PD-1) ELISA Kit
KTE70783-01ELISA kit for Mouse Programmed Death 1 (PD-1)
KTE63119-01ELISA kit for Human Programmed death-ligand 1 (PD-L1)  Kit
PPT-14462Human PDCD4/PD4 Recombinant Protein
ANT-13681-50ugPDCD4 / PD4 Polyclonal Antibody

Alternative Products

CatalogName
abx137403-01Programmed Cell Death 4 (PDCD4) Antibody
CSB-E13638h-01Human programmed cell death 4 ( PDCD4) ELISA Kit
EK8627Human Programmed cell death 4 (PDCD4) ELISA Kit
TRV-055209-01ELISA Kit for Programmed Cell Death Protein 4 (PDCD4)
G1643Programmed cell death 4 (PDCD4) Human ELISA Kit
abx002007-01Programmed Cell Death Protein 4 (PDCD4) Antibody
abx616842Programmed Cell Death Protein 4 (PDCD4) Peptide
JOT-EK4077hu-01Human Programmed cell death protein 4,PDCD4 ELISA KIT
MBS2706884-01Human Programmed Cell Death Protein 4 (PDCD4) ELISA Kit
G1663Programmed cell death protein 4 (PDCD4) Mouse ELISA Kit

Popular Products