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Human Complement factor B precursor (pre-CFB) ELISA Kit

CAT: 0749-KTE61176-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0749-KTE61176-01Size:48 Tests
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Background
CFB encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. CFB localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in this gene are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of this gene is 421 bp from the 5' end of CFB for complement component 2.
Description
This Human Complement factor B precursor (pre-CFB) ELISA Kit employs a two-site sandwich ELISA to quantitate pre-CFB.
CAS Number
7732-18-5
Product Name Alternative
Pre-CFB
Reactivity
Human
Tag
Pre-CFB
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Complement factor B precursor (pre-CFB) ELISA Kit has high sensitivity and excellent specificity for detection of Human pre-CFB. No significant cross-reactivity or interference between Human pre-CFB and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Human Complement factor B precursor (pre-CFB) ELISA Kit employs a two-site sandwich ELISA to quantitate pre-CFB in samples. An antibody specific for pre-CFB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anypre-CFB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pre-CFB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pre-CFB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
Pre-CFB
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Calibration Range
Please inquire

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