Human Protein Tob1 (TOB1) ELISA Kit

CAT: 0749-KTE60171-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0749-KTE60171-01Size:48 Tests
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Background
TOB1 encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.
Description
This Human Protein Tob1 (TOB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOB1.
CAS Number
7732-18-5
Product Name Alternative
TOB1; APRO6; MGC104792; MGC34446; PIG49; TOB; TROB; TROB1; transducer of erbB-2
Gene ID
10140
Accession Number
P50616
Reactivity
Human
Tag
TOB1
Type
Cell analysis
Applications
ELISA
Detection Method
Colorimetric
Assay Type
Sandwich ELISA (quantitative)
Assay Performance Time
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Limit Of Detection
Please inquire
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Protein Tob1 (TOB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TOB1. No significant cross-reactivity or interference between Human TOB1 and analogues was observed.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Applications Notes
This Human Protein Tob1 (TOB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOB1 in samples. An antibody specific for TOB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Other Product Names
TOB1; APRO6; MGC104792; MGC34446; PIG49; TOB; TROB; TROB1; transducer of erbB-2
Tested Applications
ELISA Testing
Recommended Usage
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Accession Number URL
http://www.uniprot.org/uniprot/P50616
Calibration Range
Please inquire

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