PCNA Polyclonal Antibody

• Background:

  PCNAThe protein encoded by PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.

• Description:

  Rabbit Anti-PCNA Polyclonal Antibody

• Product Name Alternative:

  Proliferating cell nuclear antigen; PCNA; Cyclin

• Gene ID:

  5111

• Accession Number:

  P12004

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from part region of human PCNA protein at amino acid sequence of Internal

• Clonality:

  Polyclonal

• Tag:

  PCNA

• Applications:

  WB, ELISA, IHC

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  30-33kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  This antibody detects endogenous levels of PCNA

• Observed Molecular Weight:

  36

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000

• Other Product Names:

  Proliferating cell nuclear antigen; PCNA; Cyclin

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/P12004

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG