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MDHC Polyclonal Antibody

CAT: 0749-ABP59246-01Size: 30 µLDry Ice: NoHazardous: No
CAT#:0749-ABP59246-01Size:30 µL
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Background
MDH1 encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.
Description
Rabbit Anti-MDHC Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase
Gene ID
4190
Accession Number
P40925
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from part region of human MDHC protein
Clonality
Polyclonal
Tag
MDHC
Applications
WB, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
36kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
The antibody detects endogenous levels of MDHC protein
Observed Molecular Weight
36
Formulation
Liquid solution
Buffer pH
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000
Other Product Names
Malate dehydrogenase, cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P40925
Isotype
Rabbit IgG

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