GDNF Polyclonal Antibody

• Background:

  GDNF encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. The recombinant form of this protein, a highly conserved neurotrophic factor, was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. This protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Mutations in GDNF may be associated with Hirschsprung disease and Tourette syndrome. GDNF encodes multiple protein isoforms that may undergo similar proteolytic processing.

• Description:

  Rabbit Anti-GDNF Polyclonal Antibody

• Product Name Alternative:

  Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF

• Gene ID:

  2668

• Accession Number:

  P39905

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from part region of human GDNF protein at amino acid sequence of 100-180

• Clonality:

  Polyclonal

• Tag:

  GDNF

• Applications:

  WB, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  23kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  The antibody detects endogenous levels of GDNF protein

• Observed Molecular Weight:

  23

• Formulation:

  Liquid solution

• Buffer pH:

  PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000

• Other Product Names:

  Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor; ATF

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/P39905

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG