Cdc25A Polyclonal Antibody

• Background :

  CDC25A (cell division cycle 25A) is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

• Description :

  Rabbit Anti-Cdc25A Polyclonal Antibody

• Product Name Alternative :

  CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A

• Gene ID :

  993

• Accession Number :

  P30304

• Reactivity :

  Human, Mouse, Rat, Monkey

• Immunogen :

  Synthesized peptide derived from human Cdc25A around the non-phosphorylation site of S75

• Clonality :

  Polyclonal

• Tag :

  M-phase inducer phosphatase 1

• Applications :

  WB, IHC, IF, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  60kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  Cdc25A Polyclonal Antibody detects endogenous levels of Cdc25A protein.

• Observed Molecular Weight :

  60

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.

• Other Product Names :

  CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/P30304

• CAS Number :

  9007-83-4

• Isotype :

  Rabbit IgG