RIN1 Polyclonal Antibody

• Background:

  Ras is a membrane-associated small G protein that is indirectly coupled to receptor and nonreceptor tyrosine kinases. Ras activation is regulated by the levels of bound GTP and GDP. Several effectors of Ras have been identified, including Raf1, PI 3-kinase and RIN1. RIN1 (Ras interaction/interference) was identified as a Ras-interacting protein in yeast, and it has been shown to bind to the human H-Ras. This RIN1-Ras interaction is enhanced when Ras is bound to GTP. Unlike Raf1, RIN1 is localized primarily to the plasma membrane. RIN1 contains an SH2 domain and an amino-terminal region similar to consensus SH3 domains. RIN1 binds c-Abl and, like Raf1, interacts with 14-3-3 proteins.

• Description:

  Rabbit Anti-RIN1 Polyclonal Antibody

• Product Name Alternative:

  RIN1; Ras and Rab interactor 1; Ras inhibitor JC99; Ras interaction/interference protein 1

• Gene ID:

  9610

• Accession Number:

  Q13671

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from the C-terminal region of human RIN1 at AA range: 630-710

• Clonality:

  Polyclonal

• Tag:

  Ras and Rab interactor 1

• Applications:

  WB, ELISA, IHC

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  84kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  RIN1 Polyclonal Antibody detects endogenous levels of RIN1 protein.

• Observed Molecular Weight:

  84

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000

• Other Product Names:

  RIN1; Ras and Rab interactor 1; Ras inhibitor JC99; Ras interaction/interference protein 1

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/Q13671

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG