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MYPT3 Polyclonal Antibody

CAT: 0749-ABP56794-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP56794-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Myosin light chain kinase and phosphatase (MLCP) complexes control the phosphorylation states of regulatory myosin light chains, which is crucial for muscle and intracellular movement. MLCPs typically contain a catalytic protein phosphatase 1 (PP1c) subunit, a myosin phosphatase targeting (MYPT) subunit, and another smaller subunit. The protein encoded by MYPT3 (protein phosphatase 1 regulatory subunit 16A, PPP1R16A) represents an MYPT subunit, which is responsible for directing PP1c to its intended targets. However, while other MYPTs result in PP1c activation after becoming phosphorylated, the encoded protein is phosphorylated by protein kinase A and then inhibits the catalytic activity of PP1c.
Description
Rabbit Anti-MYPT3 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
PPP1R16A; MYPT3; Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3
Gene ID
84988
Accession Number
Q96I34
Reactivity
Human, Mouse
Immunogen
Synthesized peptide derived from the Internal region of human MYPT3 at AA range: 340-420
Clonality
Polyclonal
Tag
Protein phosphatase 1 regulatory subunit 16A
Applications
WB, IF, ELISA, IHC
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
60kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
MYPT3 Polyclonal Antibody detects endogenous levels of MYPT3 protein.
Observed Molecular Weight
60
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IF/ICC1:50-200; ELISA 1:2000-20000; IHC 1:50-200
Other Product Names
PPP1R16A; MYPT3; Protein phosphatase 1 regulatory subunit 16A; Myosin phosphatase-targeting subunit 3
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q96I34
Isotype
Rabbit IgG

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