C-Src (phospho Ser75) Polyclonal Antibody

• Background:

  SRC (SRC proto-oncogene, non-receptor tyrosine kinase) is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by SRC is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for SRC.

• Description:

  Rabbit Anti-c-Src (phospho Ser75) Polyclonal Antibody

• Product Name Alternative:

  SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

• Gene ID:

  6714

• Accession Number:

  P12931

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human c-Src around the phosphorylation site of S75

• Clonality:

  Polyclonal

• Tag:

  Proto-oncogene tyrosine-protein kinase Src

• Applications:

  WB, IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  60kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  Phospho-c-Src (S75) Polyclonal Antibody detects endogenous levels of c-Src protein only when phosphorylated at S75.

• Observed Molecular Weight:

  60

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200

• Other Product Names:

  SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/P12931

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG