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PKC ε Polyclonal Antibody

CAT: 0749-ABP55782-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP55782-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by PRKCE (protein kinase C epsilon) is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS) -mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
Description
Rabbit Anti-PKC ε Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
PRKCE; PKCE; Protein kinase C epsilon type; nPKC-epsilon
Gene ID
5581
Accession Number
Q02156
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human PKC ε around the non-phosphorylation site of S729
Clonality
Polyclonal
Tag
Protein kinase C epsilon type
Applications
WB, ELISA, IHC
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
83kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
PKC ε Polyclonal Antibody detects endogenous levels of PKC ε protein.
Observed Molecular Weight
83
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:50-300; ELISA 1:2000-20000
Other Product Names
PRKCE; PKCE; Protein kinase C epsilon type; nPKC-epsilon
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q02156
Isotype
Rabbit IgG

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