CPLA2 Polyclonal Antibody

• Background :

  PLA2G4A encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca (2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants.

• Description :

  Rabbit Anti-cPLA2 Polyclonal Antibody

• Product Name Alternative :

  PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA

• Gene ID :

  5321

• Accession Number :

  P47712

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthesized peptide derived from the N-terminal region of human cPLA2

• Clonality :

  Polyclonal

• Tag :

  Cytosolic phospholipase A2

• Applications :

  WB, IHC, IF, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  114kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  CPLA2 Polyclonal Antibody detects endogenous levels of cPLA2 protein.

• Observed Molecular Weight :

  114

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-300; ELISA 1:20000; IF 1:50-200

• Other Product Names :

  PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/P47712

• CAS Number :

  9007-83-4

• Isotype :

  Rabbit IgG