α-SMA Polyclonal Antibody

• Background:

  Actin aortic smooth muscle encoded by ACTA2 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

• Description:

  Rabbit Anti-α-SMA Polyclonal Antibody

• Product Name Alternative:

  ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

• Gene ID:

  59

• Accession Number:

  P62736

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from the N-terminal region of human α-SMA

• Clonality:

  Polyclonal

• Tag:

  Actin aortic smooth muscle

• Applications:

  WB, IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  42kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  α-SMA Polyclonal Antibody detects endogenous levels of α-SMA protein.

• Observed Molecular Weight:

  42

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200

• Other Product Names:

  ACTA2; ACTSA; ACTVS; GIG46; Actin; aortic smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/P62736

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG