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P27 Polyclonal Antibody

CAT: 0749-ABP52104-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP52104-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
CDKN1B encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. Cyclin dependent kinase inhibitor 1B binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of cyclin dependent kinase inhibitor 1B, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in CDKN1B are associated with multiple endocrine neoplasia type IV (MEN4) .
Description
Rabbit Anti-p27 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Gene ID
1027
Accession Number
P46527
Reactivity
Human, Rat, Mouse, Bovine
Immunogen
Synthesized peptide derived from human p27 around the non-phosphorylation site of T198
Clonality
Polyclonal
Tag
Cyclin-dependent kinase inhibitor 1B
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
27kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
P27 Polyclonal Antibody detects endogenous levels of p27 protein.
Observed Molecular Weight
27
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:10000-20000; IHC 1:50-300; IF 1:50-200
Other Product Names
CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P46527
Isotype
Rabbit IgG

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