DIO3 Polyclonal Antibody

• Background :

  Deiodinase, iodothyronine, type III encoded by this intronless gene (DIO3) belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

• Description :

  Rabbit Anti-DIO3 Polyclonal Antibody

• CAS Number :

  9007-83-4

• Product Name Alternative :

  DIO3; ITDI3; TXDI3; Type III iodothyronine deiodinase; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase

• Gene ID :

  1735

• Accession Number :

  P55073

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthesized peptide derived from the Internal region of human DIO3

• Clonality :

  Polyclonal

• Tag :

  Type III iodothyronine deiodinase

• Applications :

  WB, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  31kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  DIO3 Polyclonal Antibody detects endogenous levels of DIO3 protein.

• Observed Molecular Weight :

  31

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications.

• Other Product Names :

  DIO3; ITDI3; TXDI3; Type III iodothyronine deiodinase; 5DIII; DIOIII; Type 3 DI; Type-III 5'-deiodinase

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/P55073

• Isotype :

  Rabbit IgG