CD5 Polyclonal Antibody

• Background :

  CD5 (also designated Lyt-1) has been identified as a transmembrane glycoprotein that is expressed on 70% of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/ CD3-z chain and with the Src family kinase Lck p56. In vitro studies have shown a 10- to 15-fold increase in the kinase activity of Lck bound to CD5. The B cell antigen, CD72, serves as a receptor for CD5. The consequence of CD5 binding to its cognate receptor is still in question and likely plays a role in thymic selection.

• Description :

  Rabbit Anti-CD5 Polyclonal Antibody

• CAS Number :

  9007-83-4

• Product Name Alternative :

  CD5; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD antigen CD5

• Gene ID :

  921

• Accession Number :

  P06127

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Synthesized peptide derived from human CD5 around the non-phosphorylation site of Y453

• Clonality :

  Polyclonal

• Tag :

  T-cell surface glycoprotein CD5

• Applications :

  WB, ELISA

• Purification Method :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration :

  1 mg/ml

• Molecular Weight :

  60kD

• Precautions :

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions :

  Gel pack with blue ice.

• Storage Conditions :

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes :

  CD5 Polyclonal Antibody detects endogenous levels of CD5 protein.

• Observed Molecular Weight :

  60

• Formulation :

  Liquid solution

• Buffer pH :

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes :

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications.

• Other Product Names :

  CD5; LEU1; T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; CD antigen CD5

• Tested Applications :

  WB, IHC, IF, IP, ELISA

• Host or Source :

  Rabbit

• Accession Number URL :

  http://www.uniprot.org/uniprot/P06127

• Isotype :

  Rabbit IgG