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FLAG-tag Polyclonal Antibody

CAT: 0749-ABP50079-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP50079-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Description
Rabbit Anti-FLAG-tag Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope
Reactivity
Species independent
Immunogen
DDDDK synthetic peptide conjugated to KLH
Clonality
Polyclonal
Tag
FLAG
Applications
WB, ELISA, IP, IF
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-1:3000; ELISA 1:5000-20000; IF 1:50-200
Other Product Names
DDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Isotype
Rabbit IgG

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