PCNA rabbit pAb

• Background:

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

• Description:

  The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008],

• UniProt:

  P12004

• Swiss Prot:

  P12004

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  Synthesized peptide derived from human PCNA. at AA range: Internal

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; ELISA; IHC

• Concentration:

  1 mg/ml

• Dilution:

  WB 1:500-2000; IHC-p 1:50-300; ELISA 2000-20000

• Molecular Weight:

  30-33kd

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  30-33kd

• Fragment:

  IgG

• Subcellular Location:

  Nucleus . Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902) . Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase (PubMed:15543136) . Co-localizes with SMARCA5/SNF2H and BAZ1B/WSTF at replication foci during S phase (PubMed:15543136) . Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. .

• Other Product Names:

  Proliferating cell nuclear antigen (PCNA) (Cyclin)

• Gene ID (Human):

  5111