MELK rabbit pAb

• Background:

  Catalytic activity:ATP + a protein = ADP + a phosphoprotein., function:Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis., PTM:Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily., similarity:Contains 1 KA1 (kinase-associated) domain., similarity:Contains 1 protein kinase domain., subunit:Interacts with ZNF622 and PPP1R8., tissue specificity:Expressed in placenta, kidney, thymus, testis, ovary and intestine.

• Description:

  Catalytic activity: ATP + a protein = ADP + a phosphoprotein. function: Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis. PTM: Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. similarity: Contains 1 KA1 (kinase-associated) domain. similarity: Contains 1 protein kinase domain. subunit: Interacts with ZNF622 and PPP1R8. tissue specificity: Expressed in placenta, kidney, thymus, testis, ovary and intestine.

• UniProt:

  Q14680

• Swiss Prot:

  Q14680

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human MELK. AA range:431-480

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  75kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  75kD

• Fragment:

  IgG

• Subcellular Location:

  Cell membrane ; Peripheral membrane protein .

• Other Product Names:

  MELK; KIAA0175; Maternal embryonic leucine zipper kinase; hMELK; Protein kinase Eg3; pEg3 kinase; Protein kinase PK38; hPK38; Tyrosine-protein kinase MELK

• Gene ID (Human):

  9833