Vinculin (phospho Tyr821) rabbit pAb

Vinculin (phospho Tyr821) rabbit pAb

• Description: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],
• Synonyms: VCL; Vinculin; Metavinculin
• Gene ID: 7414
• UniProt: P18206
• Cellular Locus: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side. Cell junction, adherens junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions..
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821. AA range:786-835
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.