53BP1 (phospho Ser6) rabbit pAb

• Background:

  Function:May have a role in checkpoint signaling during mitosis (By similarity) . Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage., PTM:Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding., PTM:Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation., similarity:Contains 2 BRCT domains., subcellular location:Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks., subunit:Interacts with IFI202A (By similarity) . Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro) . Does not bind unmethylated histone H3.

• Description:

  Function: May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage. PTM: Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding. PTM: Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation. similarity: Contains 2 BRCT domains. subcellular location: Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. subunit: Interacts with IFI202A (By similarity). Binds to the central domain of TP53/p53. May form homo-oligomers. Interacts with DCLRE1C. Interacts with histone H2AFX and this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with histone H4 that has been dimethylated at 'Lys-20'. Has low affinity for histone H4 containing monomethylated 'Lys-20'. Does not bind histone H4 containing unmethylated or trimethylated 'Lys-20'. Has low affinity for histone H3 that has been dimethylated on 'Lys-79'. Has very low affinity for histone H3 that has been monomethylated on 'Lys-79' (in vitro). Does not bind unmethylated histone H3.

• UniProt:

  Q12888

• Swiss Prot:

  Q12888

• Reactivity:

  Human; Mouse; Rat; Monkey

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser6. AA range:1-50

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight:

  213kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  213kD

• Fragment:

  IgG

• Subcellular Location:

  Nucleus . Chromosome . Chromosome, centromere, kinetochore . Localizes to the nucleus in absence of DNA damage (PubMed:28241136) . Following DNA damage, recruited to sites of DNA damage, such as double stand breaks (DSBs) : recognizes and binds histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23333306, PubMed:23760478, PubMed:24703952, PubMed:28241136, PubMed:17190600) . Associated with kinetochores during mitosis (By similarity) . .

• Other Product Names:

  TP53BP1; Tumor suppressor p53-binding protein 1; 53BP1; p53-binding protein 1; p53BP1

• Gene ID (Human):

  7158