SP-100 rabbit pAb

SP-100 rabbit pAb

• Description: This gene encodes a subnuclear organelle and major component of the PML (promyelocytic leukemia)-SP100 nuclear bodies. PML and SP100 are covalently modified by the SUMO-1 modifier, which is considered crucial to nuclear body interactions. The encoded protein binds heterochromatin proteins and is thought to play a role in tumorigenesis, immunity, and gene regulation. Alternatively spliced variants have been identified for this gene; one of which encodes a high-mobility group protein. [provided by RefSeq, Aug 2011],
• Synonyms: SP100; Nuclear autoantigen Sp-100; Lysp100b; Nuclear dot-associated Sp100 protein; Speckled 100 kDa
• Gene ID: 6672
• UniProt: P23497
• Cellular Locus: Nucleus. Nucleus, PML body. Cytoplasm. Differences in the subnuclear localization of the different isoforms seem to exist and may also be cell cycle- and interferon-dependent. Accumulates in the cytoplasm upon FAS activation.; [Isoform Sp100-C]: Nucleus. Forms a reticulate or track-like nuclear pattern with denser concentrations at the nuclear lamina and surrounding the nucleoli, a pattern reminiscent of heterochromatin-rich regions according to PubMed:11313457.
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: Synthesized peptide derived from SP-100 . at AA range: 250-330
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Molecular Weight: 100kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.