CPLA2 (phospho Ser505) rabbit pAb

• Background:

  This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca (2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

• Description:

  This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca (2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015],

• Synonyms:

  PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA

• Gene ID:

  5321

• UniProt:

  P47712

• Cellular Locus:

  Cytoplasm. Golgi apparatus membrane. Nucleus envelope. Translocates to intracellular membranes in a calcium-dependent way..

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505. AA range:471-520

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, IHC, IF, ELISA

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  110kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  110 kD

• Subcellular Location:

  Cytoplasm . Golgi apparatus membrane . Nucleus envelope. Translocates to intracellular membranes in a calcium-dependent way. .

• Other Product Names:

  PLA2G4A; CPLA2; PLA2G4; Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA

• Gene ID (Human):

  5321

• SwissProt (Human):

  P47712