ASK 1 (phospho Ser83) rabbit pAb

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CAT#:0855-ES6231-01Size:50 µL

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Background

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK) . MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK) /stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by Re

Description

Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1, 374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by Re

UniProt

Q99683

Swiss Prot

Q99683

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human ASK1 around the phosphorylation site of Ser83. AA range:49-98

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Storage Conditions

-20°C/1 year

Fragment

IgG

Subcellular Location

Cytoplasm . Endoplasmic reticulum. Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region.

Other Product Names

MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5

Gene ID (Human)

4217

UniProtKB · Q99683

Mitogen-activated protein kinase kinase kinase 5

M3K5_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q99683
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: MAP3K5
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: 2.7.11.25
Processing: —
Secondary accessions: A6NIA0, B4DGB2, Q5THN3, Q99461

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisHost-virus interactionImmunityInnate immunityStress response

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Domain

Coiled coil

Cellular component

CytoplasmEndoplasmic reticulum

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

MethylationPhosphoproteinUbl conjugation

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