MDM2 (phospho Ser186/S188) rabbit pAb

MDM2 (phospho Ser186/S188) rabbit pAb

• Description: This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells. [provided by RefSeq, Jun 2013],
• Synonyms: MDM2; E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2
• Gene ID: 4193
• UniProt: Q00987
• Cellular Locus: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Nucleus. Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: The antiserum was produced against synthesized peptide derived from human MDM2 around the phosphorylation site of Ser186 and Ser188. AA range:151-200
• Clonality: Polyclonal
• Validated Applications: IF, WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:500-2000 Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.