c-Abl (phospho Tyr245) rabbit pAb

c-Abl (phospho Tyr245) rabbit pAb

• Description: This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t (9;22) translocation that results in a fusion with the 5' end of the breakpoint cluster region gene (BCR; MIM: 151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. [pr
• Synonyms: ABL1; ABL; JTK7; Tyrosine-protein kinase ABL1; Abelson murine leukemia viral oncogene homolog 1; Abelson tyrosine-protein kinase 1; Proto-oncogene c-Abl; p150
• Gene ID: 25
• UniProt: P00519
• Cellular Locus: Cytoplasm, cytoskeleton. Nucleus. Mitochondrion. Shuttles between the nucleus and cytoplasm depending on environmental signals. Sequestered into the cytoplasm through interaction with 14-3-3 proteins. Localizes to mitochondria in response to oxidative stress (By similarity)..; [Isoform IB]: Nucleus membrane; Lipid-anchor. The myristoylated c-ABL protein is reported to be nuclear.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human c-Abl around the phosphorylation site of Tyr245. AA range:196-245
• Clonality: Polyclonal
• Validated Applications: WB, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Molecular Weight: 125(200kd BCR-ABL complex)
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.