IL-1β rabbit pAb

• Background:

  The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE) . This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]

• Description:

  The protein encoded by this gene is a member of the interleukin 1 Cytokine family. This Cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This Cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this Cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a Cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008],

• UniProt:

  P01584

• Swiss Prot:

  P01584

• Reactivity:

  Human; Mouse; Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from the Internal region of human IL1B. AA range:181-230

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

• Molecular Weight:

  17kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  17kD

• Fragment:

  IgG

• Subcellular Location:

  Cytoplasm, cytosol . Secreted . Lysosome . Secreted, extracellular exosome . The precursor is cytosolic (PubMed:15192144) . In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted (PubMed:24201029, PubMed:33377178, PubMed:33883744) . Mature form is secreted and released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore (PubMed:33883744) . In contrast, the precursor form is not released, due to the presence of an acidic region that is proteolytically removed by CASP1 during maturation (PubMed:33883744) . The secretion is dependent on protein unfolding and facilitated by the cargo receptor TMED10 (PubMed:32272059) . .

• Other Product Names:

  IL1B; IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin

• Gene ID (Human):

  3553