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Ref-1 rabbit pAb

CAT: 0855-ES3337-02Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0855-ES3337-02Size:100 µL
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Background
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]
Description
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008],
UniProt
P27695
Swiss Prot
P27695
Reactivity
Human; Mouse; Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human APEX1. AA range:191-240
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC; IF; ELISA
Concentration
1 mg/ml
Dilution
Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
Molecular Weight
34kD
Storage Conditions
-20°C/1 year
Observed Molecular Weight
34kD
Fragment
IgG
Subcellular Location
Nucleus. Nucleus, nucleolus. Nucleus speckle. Endoplasmic reticulum. Cytoplasm. Detected in the cytoplasm of B-cells stimulated to switch (By similarity) . Colocalized with SIRT1 in the nucleus. Colocalized with YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is recruited to nuclear speckles in UVA-irradiated cells. Colocalized with nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle dependent and requires active rRNA transcription. Colocalized with calreticulin in the endoplasmic reticulum. Translocation from the nucleus to the cytoplasm is stimulated in presence of nitric oxide (NO) and function in a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export signal (amino acid position 64-80) . S-nitrosylation at Cys-93 and
Other Product Names
APEX1; APE; APE1; APEX; APX; HAP1; REF1; DNA- (apurinic or apyrimidinic site) lyase; APEX nuclease; APEN; Apurinic-apyrimidinic endonuclease 1; AP endonuclease 1; APE-1; REF-1; Redox factor-1
Gene ID (Human)
328

UniProtKB · P27695

DNA repair nuclease/redox regulator APEX1

APEX1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P27695
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APEX1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.11.2, 3.1.21.-
Processing
—
Secondary accessions
Q969L5, Q99775
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationCleavage on pair of basic residuesDisulfide bondPhosphoproteinS-nitrosylationUbl conjugation

Molecular function

ActivatorDNA-bindingEndonucleaseExonucleaseHydrolaseNucleaseRepressorRNA-binding

Cellular component

CytoplasmEndoplasmic reticulumMitochondrionNucleus

Biological process

DNA damageDNA recombinationDNA repairTranscriptionTranscription regulation

Ligand

MagnesiumMetal-binding

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