HSF1 rabbit pAb

• Background:

  Heat shock transcription factor 1 (HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE) . This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016]

• Description:

  Heat shock transcription factor 1 (HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016],

• UniProt:

  Q00613

• Swiss Prot:

  Q00613

• Reactivity:

  Human; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human HSF1. AA range:270-319

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  82kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  82kD

• Fragment:

  IgG

• Subcellular Location:

  Nucleus . Cytoplasm . Nucleus, nucleoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920) . Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787) . Translocates in the nucleus upon heat shock (PubMed:8455624) . Nucleocytoplasmic shuttling protein (PubMed:26159920) . Colocalizes with IER5 in the nucleus (PubMed:27354066) . Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920) . Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:1151455

• Other Product Names:

  HSF1; HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1

• Gene ID (Human):

  3297