HIF-1α rabbit pAb

HIF-1α rabbit pAb

• Description: Hypoxia inducible factor 1 alpha subunit (HIF1A) Homo sapiens This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011],
• Synonyms: HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma
• Gene ID: 3091
• UniProt: Q16665
• Cellular Locus: Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with HIF3A in the nucleus and speckles (By similarity). Cytoplasmic in normoxia, nuclear translocation in response to hypoxia (PubMed:9822602)..
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377
• Clonality: Polyclonal
• Validated Applications: IF, WB, IHC, IP, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000.IP 1:200 Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Molecular Weight: 92-130kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.