DDDDK-Tag (binds to flag sequnence) rabbit pAb

DDDDK-Tag (binds to flag sequnence) rabbit pAb

• Description: The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
• Synonyms: DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag
• Host: Rabbit
• Species Reactivity: Panspecies
• Immunogen: DDDDK synthetic peptide conjugated to KLH.
• Clonality: Polyclonal
• Validated Applications: WB, ELISA, IP, IF
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1:5000-20000. Not yet tested in other applications.
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.