Mouse TF (Tissue factor) ELISA Kit

CAT: 0763-E-EL-M1163-01Size: 24 TestsDry Ice: NoHazardous: No

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CAT#:0763-E-EL-M1163-01Size:24 Tests

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Abbreviation

UniProt

P20352

Cross Reactivity

This kit recognizes Mouse TF in samples. No significant cross-reactivity or interference between Mouse TF and analogues was observed

Target

F3; FIII; CD142; TFA; Thromboplastin; Coagulation Factor III

Type

Sandwich-ELISA

Applications

ELISA

Field of Research

Neuroscience

Assay Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TF. You can calculate the concentration of Mouse TF in the samples by comparing the OD of the samples to the standard curve.

Assay Performance Time

3 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

6.25-400 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

3.75 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Species

Mouse

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Mouse TF concentrations in serum, plasma and other biological fluids.

UniProtKB · P20352

Tissue factor

TF_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: P20352
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: F3
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: TF
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Biological process

Blood coagulationHemostasis

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Cellular component

Membrane

Technical term

Reference proteome

Domain

SignalTransmembraneTransmembrane helix

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