Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Tetramer Protein (MALS verified)

Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Tetramer Protein (MALS verified)

• Background: The MAGE A4 antigen is a cancer-testis antigen and is expressed intracellularly in various solid tumor tissues, MAGE A4230-239 peptide (GVYDGREHTV) is a cytotoxic T lymphocyte (CTL) epitope presented by HLA-A2 The Human HLA-A*0201 MAGE-A4 (GVYDGREHTV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GVYDGREHTV peptide of the MAGE-A4.
• Description: Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Tetramer Protein (HLK-H52E5) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & KVLEHVVRV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & KVLEHVVRV).
• Specifications: Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Tetramer Protein is assembled by biotinylated monomer and streptavidin. Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein is produced by co-expression of HLA and B2M loaded with MAGE-A4 peptide. Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa, 13.3 kDa and 11.7 kDa. The protein migrates as 40-45 kDa, 15 kDa and 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & KVLEHVVRV peptide
• Host: HEK 293
• Target: HLA-A*02:01 & B2M & MAGE-A4 (KVLEHVVRV)
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human HLA-A*02:01&B2M&MAGE-A4 (KVLEHVVRV) Tetramer Protein (Cat. No. HLK-H52E5) at 1 μg/mL (100 μL/well) can bind Anti-B2M Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (Routinely tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.3 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C