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Maltose Phosphorylase Activity Kit (Fluorometric)

CAT: 0026-K353-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K353-100Size:100 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Maltose phosphorylase (EC 2.4.1.8) belongs to the family of hexosyltransferases. It catalyzes maltose and inorganic phosphate to produce glucose and glucose-1-phosphate (G1P) during Maltose metabolism. Maltose phosphorylase is commonly identified in gram positive and gram negative bacteria including E. coli, Bacillus subtilis, Enterococcus sp. and Lactobacillus brevis etc. BioVision’s Maltose Phosphorylase Activity Assay Kit is the first commercial assay to determine the Maltose Phosphorylase activity in different bacterial lysates. The assay converts maltose to produce Glucose, which is then detected by a set of enzymatic reactions to generate a fluorescent product with a Ex/Em 535/587 nm. The fluorescence signal is directly proportional to the Maltose Phosphorylase activity. BioVision’s Matlose Phosphorylase Activity Assay Kit is rapid, sensitive and a convenient tool for detecting Maltose Phosphorylase activity. It can detect as low as 0.5 mU under the assay conditions.
Reactivity
Bacterial lysates
Applications
Analysis of Maltose Phosphorylase kinetics in inhibition or activation screening, Measurement of Maltose Phosphorylase activity in different bacteria
Detection Method
Fluorescence (Ex/Em 535/587 nm)
Features & Benefits
Sensitive, Rapid, Convenient tool for detecting Maltose Phosphorylase activity, It can detect as low as 0.5 mU under the assay conditions
Shipping Conditions
Gel Pack
Storage Conditions
-20°C
Shelf Life
12 months

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