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AMP Colorimetric Assay Kit

CAT: 0026-K229-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-K229-100Size:100 Assays
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Description
Adenosine Monophosphate (AMP), also known as 5’-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories. BioVision’s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.
Reactivity
All
Applications
Study the regulation of AMPK by AMP, Mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc., Measurement of AMP level in various cell or tissue types
Detection Method
Absorbance, OD 570 nm
Shipping Conditions
Gel Pack
Storage Conditions
-20°C
Shelf Life
12 months

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