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Brucella IgM ELISA Kit

CAT: 0026-E4710-100Size: 100 AssaysDry Ice: NoHazardous: No
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CAT#:0026-E4710-100Size:100 Assays
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Description
Brucella is a gram negative coccobacilli capable of infecting a wide range of animal and man. Of the three species causing human infection, B. melitensis is the most patogenic followed by B. suis and B. abortus . Brucellosis is transmitted through contaminated and untreated milk and milk products and by direct contact with infected animals (cattle, sheep, goats, pigs, camels, buffaloes, and, very recently, seals), animal carcasses, and abortion materials. BioVision's ELISA kit is for the detection of Brucella IgM antibody in human serum. Diluted patient serum (serum diluent contains sorbent to remove Rheumatoid Factor and human IgG interference) is added to wells coated with purified antigen. IgM specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex if present. Excess enzyme conjugate is washed off and the substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgM specific antibody in the sample.
CAS Number
7732-18-5
Reactivity
Human
Detection Method
Absorbance-450 nm
Shipping Conditions
Gel Pack
Storage Conditions
4ºC
Shelf Life
12 months

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