QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit

QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit

• Description: BioVision's 5-lipoxygenase ELISA kit is based on Sandwich ELISA method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-LOX. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-LOX is added to each Microelisa stripplate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain 5-LOX and HRP conjugated 5-LOX antibody will appear blue in color and turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-LOX. The concentration of 5-LOX in the samples can be calculated by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Reactivity: Human
• Applications: Detection range: 1U/L - 480 U/L, Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%, CV (%) = SD/mean X 100, Sensitivity: 1U/L
• Detection Method: Absorbance-450 nm
• Features & Benefits: Detection of 5-lipoxygenase in human serum, plasma, culture media samples., Assay range 1U/L - 480 U/L, Sensitivity： 1U/L
• Shipping Conditions: Gel Pack
• Storage Conditions: 4°C
• Shelf Life: 12 months