Recombinant Mouse Hepcidin (Hamp), partial

CAT: 0399-CSB-EP887650MOe0-01Size: 10 µgDry Ice: NoHazardous: No

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CAT#:0399-CSB-EP887650MOe0-01Size:10 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Abbreviation

Recombinant Mouse Hamp protein, partial

Gene Name

Hamp

UniProt

Q9EQ21

Expression Region

59-83aa

Organism

Mus musculus (Mouse)

Target Sequence

DTNFPICIFCCKCCNNSQCGICCKT

Tag

N-terminal GST-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Others

Relevance

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

29.4 kDa

References & Citations

"Severe iron deficiency anemia in transgenic mice expressing liver hepcidin." Nicolas G., Bennoun M., Porteu A., Mativet S., Beaumont C., Grandchamp B., Sirito M., Sawadogo M., Kahn A., Vaulont S. Proc Natl Acad Sci U S A 99:4596-4601 (2002)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

UniProtKB · Q9EQ21

Hepcidin

HEPC_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: Q9EQ21
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Hamp
Protein existence: 2: Evidence at transcript level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bond

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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