Recombinant Lampetra fluviatilis NADH-ubiquinone oxidoreductase chain 1 (MT-ND1)

Recombinant Lampetra fluviatilis NADH-ubiquinone oxidoreductase chain 1 (MT-ND1)

• CAS Number: 9000-83-3
• Gene Name: MT-ND1
• UniProt: O21069
• Expression Region: 1-321aa
• Organism: Lampetra fluviatilis (European river lamprey) (Petromyzon fluviatilis)
• Target Sequence: MLVMLTSTLILVLMVLLAVAFLTMVERKTLGYMQLRKGPNVVGFMGLLQPIADGVKLFLKEPVWPTAASPALFIAAPIMALTLALSLWMFIPMPQSISTINLTLLVILAISSLSVYASLGSGWASNSKYALIGALRAVAQTISYEVSLGLILLCLIILTGGFSLQAFIYTQEHTWFLLSSWPLAAMWFVSTLAETNRTPFDLTEGESELVSGFNVEYAGGPFALFFLAEYSNILFMNTLTAIMFLGPLGPNNLNILPIINVMMKATPLIILFLWIRASYPRFRYDQLMHLMWKNFLPLNLALFTLQLSLAVSLGGAGVPQM
• Tag: N-terminal 10xHis-SUMO-tagged
• Source: in vitro E.coli expression system
• Field of Research: Others
• Assay Type: CF Transmembrane Protein & Developed Protein
• Relevance: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
• Purity: Greater than 85% as determined by SDS-PAGE.
• Activity: Not Test
• Length: Full Length
• Form: Liquid or Lyophilized powder
• Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).
• Molecular Weight: 53.8 kDa
• References & Citations: "The main features of the craniate mitochondrial DNA between the ND1 and the COI genes were established in the common ancestor with the lancelet." Delarbre C., Barriel V., Tillier S., Janvier P., Gachelin G. Mol. Biol. Evol. 14:807-813 (1997)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.