Succinyl-CoA Synthetase (SCS) Activity Assay Kit

Succinyl-CoA Synthetase (SCS) Activity Assay Kit

• Field of Research: Glycolysis and Lipid Metabolism;Tricarboxylic Acid（TCA）Cycle
• Detection Method: Colorimetric method
• Assay Principle: Succinyl-CoA synthetase (SCS) is a key enzyme in the citric acid cycle and an important metabolic intermediate in the biosynthesis of porphyrins, hemes, and ketones. SCS converts succinyl-coenzyme A (CoA-SH) to succinic acid. The reaction between CoA-SH and pyruvate produces chromogenic substances with a characteristic absorption peak at 450 nm. The activity of SCS can be calculated by measuring the change of absorbance value at 450 nm.
• Assay Performance Time: 60 min
• Detection Range: 0.09-20.72 U/L
• Sensitivity: 0.09 U/L
• Shipping Conditions: Ice bag
• Storage Conditions: This product can be stored at -20°C for 12 months with shading light.