Succinyl-CoA Synthetase (SCS) Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism; Tricarboxylic Acid (TCA) Cycle

• Assay Principle:

  Succinyl-CoA synthetase (SCS) is a key enzyme in the citric acid cycle and an important metabolic intermediate in the biosynthesis of porphyrins, hemes, and ketones. SCS converts succinyl-coenzyme A (CoA-SH) to succinic acid. The reaction between CoA-SH and pyruvate produces chromogenic substances with a characteristic absorption peak at 450 nm. The activity of SCS can be calculated by measuring the change of absorbance value at 450 nm.

• Assay Performance Time:

  60 min

• Sample Type:

  Tissue; cell

• Detection Range:

  0.09-20.72 U/L

• Sensitivity:

  0.09 U/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Inter CV:

  4.9-10.3%

• Intra CV:

  2.5-8.0%

• Detection Instrument:

  Microplate reader