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Recombinant Human Interferon alpha-2 (IFNA2) (Active)

CAT: 0399-CSB-MP360706HU-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-MP360706HU-01Size:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IFN-alpha-2; Interferon alpha-A (LeIF A 1)
Abbreviation
Recombinant Human IFNA2 protein (Active)
Gene Name
IFNA2
UniProt
P01563
Expression Region
24-188aa
Organism
Homo sapiens (Human)
Target Sequence
CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
Tag
N-terminal 10xHis-tagged
Type
Active Protein & In Stock Protein
Source
Mammalian cell
Field of Research
Cancer
Relevance
Produced by macrophages, IFN-alpha have antiviral activities.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Yes
Bioactivity
①Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/mL can bind Human IFNAR2 (CSB-MP011047HU) . The EC50 is 154.2-191.9 ng/mL. ②Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/mL can bind Anti-IFNA2 recombinant antibody (CSB-RA360706MA2HU) . The EC50 is 2.366-2.818 ng/mL.
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
22.9 kDa
References & Citations
IFNA2 p.Ala120Thr impairs the inhibitory activity of Interferon-alpha2 against the hepatitis B virus through altering its binding to the receptor. Chen C., Zhu X., Xu W., Yang F., Zhang G., Wu L., Zheng Y., Gao Z., Xie C., Peng L. Antiviral Res 147:11-18 (2017)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein

UniProtKB · P01563

Interferon alpha-2

IFNA2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01563
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNA2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-alpha-2
EC number
—
Processing
Precursor
Secondary accessions
H2DF54, H2DF55, P01564, Q14606, Q6DJX8, Q96KI6
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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