Recombinant Fibronectin fragment, premium grade

Recombinant Fibronectin fragment, premium grade

• Background: Fibronectin (Fn) is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Each monomer consists of three repeating units: 12 Type I, 2 Type II, and 15–17 Type III domains which combined account for 90% of the FN sequence. The extracellular matrix (ECM) plays a key role as both structural scaffold and regulator of cell signal transduction in tissues. Fibronectin is one of the major ECM proteins in the trabecular meshwork (TM). It is found in the sheath material surrounding the elastin tendons that enter the TM from the ciliary muscle within the ciliary body. In times of ECM assembly and turnover, cells upregulate assembly of the ECM protein, FN. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. These fibrils play a key role in assembling a provisional ECM during embryonic development and wound healing. Fibril assembly is also often upregulated during disease states, including cancer and fibrotic diseases.
• Description: Recombinant Fibronectin fragment, premium grade (FIN-H5116) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # P02751-15).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
• CAS Number: 9000-83-3
• Expression Region: Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107
• Host: E. coli
• Target: Fibronectin
• Conjugation: Unconjugated
• Tag: Native
• Source: Human
• Stability: -20°C±10°C for 12 months under sterile conditions.
• Endotoxin: 0.01 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Recombinant Fibronectin fragment, premium grade (Cat. No. FIN-H5116) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 0.002-0.078 μg/mL (QC tested).
• Format: Liquid
• Buffer: 12.5 mM Citric acid, pH6.2
• Additives: Glycerol
• Molecular Weight: 62.6 kDa
• Shipping Conditions: Dry ice
• Storage Conditions: -20°C