Enterokinase

• Description:

  Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.

• Expression System:

  Yeast

• Nature:

  Recombinant

• Applications:

  Fusion Protein Cleavage, Protein Purification, Protein Digestion

• Stability:

  Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

• Endotoxin:

  Please contact with the lab for this information.

• Purity:

  >90% as determined by SDS-PAGE.

• Bioactivity:

  At 25 ° C, reaction buffer 50 mM Tris-HCl (PH 8.0), the amount of enzyme required to digest 50 ug fusion protein containing the digestion site within 16 hours is defined as 1 unit of enzyme activity, which is 1U.

• Form:

  Liquid

• Buffer:

  50 mM Tris-HCl, 50%Glycerol, pH 8.0

• Shipping Conditions:

  In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

• Notes:

  For research use only.

• Species:

  Bovine