GMP Human TNF-alpha Protein

GMP Human TNF-alpha Protein

• Background: Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimers disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
• Description: GMP Human TNF-alpha Protein (GMP-TNAH23) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P01375).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 17.4 kDa. The protein migrates as 17 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Val 77 - Leu 233
• Host: HEK 293
• Target: TNF-alpha
• Conjugation: Unconjugated
• Tag: Native
• Source: Human
• Stability: -20°C to -70°C for 5 years in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution._x000D_ Upon receipt, store it immediately at -20°C or lower for long term storage.
• Endotoxin: 10 EU/mg
• Purity: 95%
• Format: Powder-GMP
• Buffer: PBS, pH7.4
• Molecular Weight: 17.4 kDa
• Shipping Conditions: Blue ice
• Storage Conditions: -20°C