SHP2 (Human) ELISA Kit

Src homology region 2 (SH2) domain-containing protein tyrosine phosphatase-2 (SHP2) is a non-receptor phosphotyrosine phosphatase. Ubiquitous expression of SHP2 is observed in a variety of cells and tissues. It is primarily localized in the cytoplasm but may also be present in other organelles (nucleus, mitochondria). SHP-2 is involved in multiple signal transduction pathways activated by hormones, cytokines, growth factors, and extracellular matrix proteins. Additionally, SHP2 plays a significant role in cell survival, differentiation, growth, and migration. When in the nucleus, SHP2 promotes DNA damage-induced apoptosis. SHP-2 mutations are associated with developmental disorders such as Noonan syndrome and LEOPARD syndrome. SHP2 is also involved in the pathogenesis of other diseases such as neurodegenerative, skeletal, metabolic diseases, and cancer. Thus, SHP-2 could serve as an efficient therapeutic target for the treatment of human diseases. BioVision’s SHP2 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of SHP2 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of SHP2 in the sample or standard

7732-18-5

Human

This Sandwich ELISA kit is designed to quantitatively measure amount of SHP2 in human serum, plasma, tissue lysates, and other biological fluids

Absorbance (450 nm)

This Sandwich ELISA is highly sensitive and highly specific for the detection of SHP2 in human samples. There is no significant cross-reactivity or interference between SHP2 and analogues,Detection range: 0.313 - 20 ng/ml,Recovery range: 85 - 105% for normal human serum and plasma samples,Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10%,Sensitivity: 0.188 ng/ml

Gel Pack

4ºC