Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (Monomer, MALS verified)

• Description:

  Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (HLM-H82Ea) is expressed from human 293 cells (HEK293) . It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388008.1 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR) .

• Specifications:

  Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with MBP peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 29.8 kDa and 29.2 kDa. The protein migrates as 37-40 kDa and 32-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  CAI2388006.1 (HLA-DRA1*01:01) & CAI2388008.1 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR

• Expression Region:

  Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR peptide

• Host:

  HEK293

• Target:

  HLA-DRA1*0101 & HLA-DRB1*1501 & MBP (ENPVVHFFKNIVTPR)

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (Cat. No. HLM-H82Ea) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.06-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  29.8 kDa and 29.2 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human