Recombinant Escherichia coli Microcin H47 (mchB)

• CAS Number:

  9000-83-3

• Gene Name:

  mchB

• UniProt:

  P62530

• Expression Region:

  16-75aa

• Organism:

  Escherichia coli

• Target Sequence:

  GGAPATSANAAGAAAIVGALAGIPGGPLGVVVGAVSAGLTTAIGSTVGSGSASSSAGGGS

• Tag:

  N-terminal 10xHis-tagged

• Source:

  in vitro E.coli expression system

• Field of Research:

  Cancer

• Assay Type:

  CF Transmembrane Protein & In Stock Protein

• Relevance:

  Bactericidal antibiotic. Active on bacteria phylogenetically related to the producing strain.

• Purity:

  Greater than 85% as determined by SDS-PAGE.

• Activity:

  Not Test

• Length:

  Full Length of Mature Protein

• Form:

  Liquid or Lyophilized powder

• Buffer:

  If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  10.9 kDa

• References & Citations:

  "The structure, function, and origin of the microcin H47 ATP-binding cassette exporter indicate its relatedness to that of colicin V." Azpiroz M.F., Rodriguez E., Lavina M. Antimicrob. Agents Chemother. 45:969-972 (2001)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.