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Anti-P-Zero Myelin Protein (PZO) Antibody

CAT: 0588-PZO-0020Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0588-PZO-0020Size:100 µL
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Background
Human Myelin Protein Zero (Pz0) is a 28,400 dalton protein (258 amino acids) found in myelin of the PNS, but not the CNS. Expressed in myelinating Schwann cells, Po serves as a homophilic adhesion molecule, allowing adjacent laminae of the myelin sheath to undergo compaction. Po is also a marker of a subpopulation of migrating neural crest cells. Mutations of the MPZ gene product in humans is responsible for Charcot-Marie-Tooth type 1B neuropathies (HMSN1B) .
Description
Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (NP_032649) and human (NP_000521) gene products. Antibodies were affinity-purified and mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.
Synonyms
Myelin protein P0, Myelin peripheral protein, MPP, Myelin protein zero
Other Statements
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Gene Name
MPZ
UniProt
O95297
Host
Chicken
Species Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, and Rat
Immunogen
Synthetic peptides
Immunogen Type
Human, Mouse, Rat
Target
Myelin Protein Zero (Pz0)
Clonality
Polyclonal
Isotype
IgY
Conjugation
Unconjugated
Applications
IHC, ICC, WB
Field of Research
Cell Markers Glial and Schwann Cell Markers
Assay Type
Primary Antibody
Assay Protocol
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Concentration
200 µg/mL
Format
Affinity-Purified IgY
Form
Affinity-Purified IgY
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Molecular Weight
27 kDa
Precautions
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Shipping Conditions
Ice Packs
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Shelf Life
12 months from receipt of product
IHC Dilution
1:2000-1:5000
WB Dilution
1:10000-1:20000
Pre Treatment
Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Po gene product, but are shared between the human (NP_000521, NCBI) and mouse (NP_032649, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
Target Description
Human Myelin Protein Zero (Pz0) is a 28,400 dalton protein (258 amino acids) found in myelin of the PNS, but not the CNS. Expressed in myelinating Schwann cells, Po serves as a homophilic adhesion molecule, allowing adjacent laminae of the myelin sheath to undergo compaction. Po is also a marker of a subpopulation of migrating neural crest cells. Mutations of the MPZ gene product in humans is responsible for Charcot-Marie-Tooth type 1B neuropathies (HMSN1B).
ICC Dilution
1:2000-1:5000
Quality Control
Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Brid
AB_2313561
Uniprot Accession Number
O95297
Physical Properties
Liquid
Isotype
IgY

UniProtKB · O95297

Myelin protein zero-like protein 1

MPZL1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O95297
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MPZL1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B2REB9, B2REC0, Q5R332, Q8IX11, Q9BWZ3, Q9NYK4, Q9UL20
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

Cellular component

Membrane
Product Datasheet
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