Anti-Prion Protein Antibody

• Background:

  Prion protein is a ubiquitously-expressed, 27661 dalton protein (253 amino acids, NP_898902.1) of unclear function (s) in eukaryotes. This protein can exist in one of two conformational forms. One is its normally-folded form (PrPc or "cellular PrP"), which contains a mixture of alpha helical regions and a few beta-pleated sheets. The other is a misfolded form (PrPsc or "scrapie PrP") whose structure contains mostly beta-pleated sheets. This misfolded scrapie PrP form is cytotoxic, especially to nervous tissue, and can serve as a "seed, " inducing adjacent, normally-folded PrP proteins to misfold into the cytotoxic conformation. In this sense, the scrapie PrP can be thought of as an infectious agent both within the organism as well between organisms.

• Description:

  Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein. After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues.

• Synonyms:

  Major prion protein, PrP, ASCR, PrP27-30, PrP33-35C, CD antigen CD230

• Other Statements:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Gene Name:

  PRNP ALTPRP PRIP PRP

• UniProt:

  P04156

• Host:

  Chicken

• Species Reactivity:

  Human, Mouse, Rat

• Reactivity:

  Human, Mouse, and Rat

• Immunogen:

  Recombinant fragment of human prion

• Immunogen Type:

  Human

• Target:

  Prion Protein (PRN)

• Clonality:

  Polyclonal

• Isotype:

  IgY

• Conjugation:

  Unconjugated

• Applications:

  IHC, ICC, WB

• Field of Research:

  Cell Markers Neuronal Markers

• Assay Type:

  Primary Antibody

• Assay Protocol:

  Chickens were immunized with a recombinant fragment of human PRN protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using the PRN protein conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

• Concentration:

  10 mg/mL

• Format:

  Mixture of IgY fraction and affinity-purified antibodies

• Form:

  Mixture of IgY fraction and affinity-purified antibodies

• Buffer:

  Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

• Molecular Weight:

  27 kDa

• Precautions:

  These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

• Shipping Conditions:

  Ice Packs

• Storage Conditions:

  Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

• Shelf Life:

  12 months from receipt of product

• IHC Dilution:

  1:1000-1:2000

• WB Dilution:

  1:2500-1:5000

• Pre Treatment:

  Chickens were immunized with a recombinant fragment of human PRN protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using the PRN protein conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

• Target Description:

  Prion protein is a ubiquitously-expressed, 27661 dalton protein (253 amino acids, NP_898902.1) of unclear function(s) in eukaryotes. This protein can exist in one of two conformational forms. One is its normally-folded form (PrPc or "cellular PrP"), which contains a mixture of alpha helical regions and a few beta-pleated sheets. The other is a misfolded form (PrPsc or "scrapie PrP") whose structure contains mostly beta-pleated sheets. This misfolded scrapie PrP form is cytotoxic, especially to nervous tissue, and can serve as a "seed," inducing adjacent, normally-folded PrP proteins to misfold into the cytotoxic conformation. In this sense, the scrapie PrP can be thought of as an infectious agent both within the organism as well between organisms.

• ICC Dilution:

  1:1000-1:2000

• Quality Control:

  Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.

• Brid:

  AB_2313559

• Uniprot Accession Number:

  P04156

• Physical Properties:

  Liquid

• Isotype:

  IgY