N-a-Tosyl-L-lysinyl-chloromethylketone (hydrochloride)

N-a-Tosyl-L-lysinyl-chloromethylketone (hydrochloride)

• CAS Number: 4238-41-9
• UNSPSC Description: N-a-Tosyl-L-lysinyl-chloromethylketone hydrochloride (TLCK hydrochloride) is an irreversible inhibitor for serine protease, which inhibits trypsin and trypsin-like protease by alkylating histidine residues in their active site. N-a-Tosyl-L-lysinyl-chloromethylketone hydrochloride inhibits caspase-3, caspase-6 and caspase-7, with IC50s of 12.0, 54.5 and 19.3 μM, respectively. N-a-Tosyl-L-lysinyl-chloromethylketone hydrochloride induces apoptosis in cell HL-60, and inhibits the decrease in mitochondrial transmembrane potential during apoptosis[1].
• Target Antigen: Caspase; Ser/Thr Protease
• Type: Reference compound
• Related Pathways: Apoptosis;Metabolic Enzyme/Protease
• Field of Research: Cancer
• Assay Protocol: https://www.medchemexpress.com/n-a-tosyl-l-lysinyl-chloromethylketone-hydrochloride.html
• Smiles: O=S(C1=CC=C(C)C=C1)(NC(C(CCl)=O)CCCCN)=O.Cl
• Molecular Weight: 369.31
• References & Citations: [1]Frydrych I, et al., Serine protease inhibitors N-alpha-tosyl-L-lysinyl-chloromethylketone (TLCK) and N-tosyl-L-phenylalaninyl-chloromethylketone (TPCK) are potent inhibitors of activated caspase proteases. J Cell Biochem. 2008 Apr 1;103(5):1646-56.
• Shipping Conditions: Room temperature